A case report of functional treatment in severe condyle resorption female adult patient

Relationship between intraarch space discrepancy and craniofacial morphology / 대한치과교정학회지

The purpose of this study was to investigate the relationship between the space discrepancy of the mandibular dentition and craniofacial morphology in adults with good Angle class I occlusion. Dental casts of normal group, crowded group and spaced group were selected on the basis of dental crowding and spacing. Subjects with excessive space to accomodate the lower teeth were classified as spaced group(39). Subjects with a space discrepancy of more than 4mm were classified as crowded group(45). Normal subjects had little or no dental crowding and spacing(40). Various skeletodental measurements in lateral cephalograms were evaluated and compared by ANOVA, Pearson correlation analysis and multiple stepwise regression analysis. The results were as follows : 1. ANB angle was larger in crowded group than in spaced group. 2. Maxilla and mandible in crowded group were inclined more downward and forward than in spaced group, so crowded group was found to have vertical tendency. 3. Anterior cranial base length and mandibular body length were longer in spaced group than in crowded group. 4. According to the multiple stepwise regression analysis with space discrepancy as dependent variable, 40% of variance of space discrepancy could be explained by ANB angle, anterior facial height and ramus height. Multiple regression equation was as follows Space discrepancy=46.51-2.51ANB-0.58AFH+0.65RH

The Use of Canine Bone-Marrow Stromal Cell Line, DO64, as Feeder Cells for The Efficient Cloning of Human B Lymphoblastoid Cell Lines ( B-LCLs ) / 대한면역학회지

Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and a tool for the establishment of human B lymphoblastoid cell lines (B-LCLs), which have proven useful for several human immunologic applications. B-LCLs serve as efficient antibody-producing cells and antigen-presenting cells. In spite of these advantages, the cloning efficiency of B-LCLs is less than 1%. In order to generate clones of B-LCLs, we cultured B-LCLs with and without canine stromal cell line, DO64, as feeder cell which was immortalized by transduction of retrovirus encoding E6 and E7 of the human papilloma virus type 16 (HPV-16), which was defined to produce various cytokines including stem cell factor (SCF) and interleukin- 6 (IL-6). After 3 weeks of B-LCLs cultured with DO64, 8.3% and 37.5% in 1 cell and 3 cells per well were efficiently cloned, respectively. There was no significant effect on growing of 8-LCLs without DO64 cells and on high concentration of FBS. The cloning efficiency of B-LCLs transduced by retrovirus cultured with and without DO64 cells was 4.2% and 0% in 3 cells per well, respectively, while that of stable transfectant 33.3% and 8.3% in 1 cell per well, respectively. Our results suggest that the use of DO64 cells as feeder cells might permit the cloning of B-LCLs. This efficient cloning of B-LCLs could be used for the convenient source of autologous antigen-presenting cells expressing foreign antigen for the study of human immune responses in vitro, and for a variety of additional purposes, such as the production of human monoclonal antibodies.